Liu et al. studied an osteoarthritis gene therapy approach using alginate hydrogels to deliver rAAV vectors carrying SOX9 and TGF-β into human osteoarthritic chondrocytes and cartilage explants. Using PHIO’s Cellwatcher M to monitor live-cell confluence and morphology, they showed sustained gene delivery, improved cartilage matrix production, and potential for future OA therapy.

Thomas et al. studied a covalent gold-based PROTAC designed to degrade cancer-relevant proteins. Using a nonproliferative HL-60 cell model, proteomics, cycloheximide chase assays, and PHIO’s Cellwatcher M to monitor cell growth, they showed selective proteasome-dependent degradation of MERTK and TXNL1.

Pause et al. studied nanoplastic uptake in farm animal cells and its influence on cellular behavior. This pilot study highlights the importance of further research into nanoplastic exposure in livestock, both to understand risks to animal health and to assess potential implications for food safety and human health.

ECP is established as a light based blood cell therapy for T-cell lymphoma and organ transplant rejection, while the full picture of how it works is still unclear. Hagn et al. identified effects of ECP on lipid mediators playing a central role in inflammation, supported by proliferation analysis of lung fibroblasts.

Reynders et al. present photoswitchable azobenzene compounds that can inhibit tubulin polymerization, allowing precise control of cell division with light. This research identifies a general method for near-quantitative photocontrol, offering potential for new anticancer therapies.

Steffens et al. present advanced nucleic acid carriers with disulfide or hydrophobic spacers. The inclusion of these spacers improved the carriers’ stability, reduced membrane damage and enhanced cell growth, meaning enhanced biocompatibility and efficacy, and proving their potential in gene therapy applications.

Mohamed et al. exploit metabolites from the soil bacterium Streptomyces mirabilis to find potential cancer drug candidates. Here they report the structure elucidation of three new peptides and their biological activities. A PHIO Cellwatcher M was used to study cancer cell growth dynamics under the influence of the compounds.

Bortel et al. uncover a memory effect of how cells were grown and handled before an experiment. Subculture quality affects the variability of the results in drug studies and may lead to globally increased variation at multiple omics levels. With tight control of culturing conditions using a Cellwatcher, the researchers could increase the predictions from omics data by a factor of 5.

ERBB2/3 are promising targets for cancer therapy. Hommel et al. studied how the deletion of these receptors affects the skin and whether their absence could explain skin-related side effects. A Cellwatcher M provided critical insights into how the loss of ERBB2 and ERBB3 affects cellular movement and wound healing processes.

Yi Lin et al. developed folate receptor–mediated Cas9 RNP nanocarriers, for the purpose of achieving efficient and specific immune checkpoint disruption in cancer cells. They used a Cellwatcher system to monitor tumor cell growth dynamics and succeeded in strongly inhibiting proliferation through dual PD-L1/PVR gene knockout.

The resistance formation of cancer cells to chemotherapeutic drugs is one of the main reasons for the failure of cancer therapy. Bianca Köhler et al. identified microRNA-200c as an important player in sensitizing cells to chemotherapeutics and reverse drug resistance. PHIO’s technology allowed for the analysis of long term effects in vitro.

Paul Stolz et al. found that stem cells are impaired in pluripotency when Tet1 is knocked out, but unaffected when only the catalytic abilities of Tet1 are suppressed. The PHIO Cellwatcher proliferation and morphology analysis showed how these genetic modifications are also reflected at the macroscopic level.

Bielesch et al. studied KRT19 in high-grade serous ovarian cancer as a marker linked to metastasis and chemoresistance. Using PHIO’s Cellwatcher M for wound healing and motility assays, they found that high KRT19 expression is associated with poorer survival, increased tumour burden, enhanced migration and adhesion, and potential paclitaxel resistance.

Zavala et al. synthesized and tested novel PST derivatives to optimize their photoresponse and compatibility with fluorescence imaging. They used Cellwatcher M to track microglial motility and showed that RJEV treatment successfully reversed pro-inflammatory mechanical and functional changes.

Bortel et al. uncover a memory effect of how cells were grown and handled before an experiment. Subculture quality affects the variability of the results in drug studies and may lead to globally increased variation at multiple omics levels. With tight control of culturing conditions using a Cellwatcher, the researchers could increase the predictions from omics data by a factor of 5.

ERBB2/3 are promising targets for cancer therapy. Hommel et al. studied how the deletion of these receptors affects the skin and whether their absence could explain skin-related side effects. A Cellwatcher M provided critical insights into how the loss of ERBB2 and ERBB3 affects cellular movement and wound healing processes.

Köhler et al. investigate the role of microRNA 200c in preventing breast cancer metastasis. The research, including Cellwatcher M cell motility analysis, shows that miR-200c hinders cancer cell motility and migration, acting as a potential metastasis suppressor and suggesting its therapeutic relevance in breast cancer treatment.

Bielesch et al. studied KRT19 in high-grade serous ovarian cancer as a marker linked to metastasis and chemoresistance. Using PHIO’s Cellwatcher M for wound healing and motility assays, they found that high KRT19 expression is associated with poorer survival, increased tumour burden, enhanced migration and adhesion, and potential paclitaxel resistance.

ERBB2/3 are promising targets for cancer therapy. Hommel et al. studied how the deletion of these receptors affects the skin and whether their absence could explain skin-related side effects. A Cellwatcher M provided critical insights into how the loss of ERBB2 and ERBB3 affects cellular movement and wound healing processes.

Köhler et al. investigate the role of microRNA 200c in preventing breast cancer metastasis. The research, including Cellwatcher M cell motility analysis, shows that miR-200c hinders cancer cell motility and migration, acting as a potential metastasis suppressor and suggesting its therapeutic relevance in breast cancer treatment.

Rempfler et al. built an end-to-end pipeline for lens-free microscopy (LFM) videos: (1) a fully-convolutional neural-network detector (ResNet-derived/UNet variants) that produces dense probability maps of cell centers; and (2) moral lineage tracing (MLTP) to jointly cluster detections and link them over time into lineage trees.

Paulitschke et al. developed an ultraflexible nanowire array, for the purpose of measuring cellular forces without labels or optical distortions. They used inverted conical semiconductor nanowires as a force sensor and succeeded in tracking cell-induced forces with piconewton to femtonewton sensitivity.